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M9480874.TXT
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1994-09-05
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Document 0874
DOCN M9480874
TI Genetic analysis of Mycobacterium avium superoxide dismutase.
DT 9410
AU Escuyer V; Haddad N; Berche P; Laboratoire de Microbiologie, INSERM,
Faculte de Medecine; Necker-Enfants Malades, Paris, France.
SO Abstr Gen Meet Am Soc Microbiol. 1994;94:176 (abstract no. U-21). Unique
Identifier : AIDSLINE ASM94/94313071
AB Mycobacterium avium is a major opportunistic pathogen widely spread in
the environment and responsible for severe infections in
immunocompromised patients with AIDS. It is able to survive and
replicate in the phagocytic cells of the host immune system and resists
the oxidative burst of activated macrophages. M. avium produces a 23-kDa
superoxide dismutase (SOD) that is thought to be involved in the
resistance to the respiratory burst-associated bactericidal mechanisms.
In order to investigate the molecular basis of this resistance, we have
cloned the gene encoding the M. avium SOD by screening a recombinant DNA
library with a probe corresponding to the SOD gene from Mycobacterium
tuberculosis. The complete nucleotide sequence of the gene was
determined. It encodes a 207 amino acid protein that shows a very high
homology with the SOD's from Mycobacterium leprae and Mycobacterium
tuberculosis (90% and 80% respectively) and very significant homology
with manganese or iron-containing SOD's from other organisms including
eucaryotes. The exact role of the M. avium SOD in intracellular survival
is now under investigation.
DE Amino Acid Sequence AIDS-Related Opportunistic
Infections/IMMUNOLOGY/MICROBIOLOGY Comparative Study *Genes, Bacterial
Human Macrophages/PHYSIOLOGY Mycobacterium avium/*ENZYMOLOGY/GENETICS
Mycobacterium leprae/ENZYMOLOGY/GENETICS Mycobacterium
tuberculosis/ENZYMOLOGY/GENETICS Phagocytosis Sequence Homology, Amino
Acid Superoxide Dismutase/*GENETICS
Tuberculosis/IMMUNOLOGY/MICROBIOLOGY MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).